ATPase activity associated with the uncoating of clathrin baskets by Hsp70.
作者： W Barouch , K Prasad , L E Greene , E Eisenberg
DOI: 10.1016/S0021-9258(19)61942-3
关键词: Clathrin 、 ATP hydrolysis 、 Auxilin 、 Hsp70 、 Vesicle 、 Biophysics 、 Biology 、 Ap180 、 Cofactor 、 Biochemistry 、 ATPase
摘要: In the presence of ATP, bovine brain hsp70 has been shown to remove clathrin from clathrin-coated vesicles in a rapid stoichiometric initial burst followed by slow steady-state uncoating. addition, it found recently that 100-kDa cofactor is required for uncoat baskets prepared with assembly protein AP-2. this study ATPase activity associated uncoating was investigated, formed and proteins. Mixed proteins or AP-2 could not be used studies because they activated even absence clathrin. However, case AP180. A ATP hydrolysis accompany AP180-clathrin hsp70, 1 mol hydrolyzed ATP/mol released heavy chain. Furthermore, needed both processes. These results suggest an occurs all baskets, accompanies uncoating, both.
sci-hub.st 参考文章(32)
B. Gao, Y. Emoto, L. Greene, E. Eisenberg, Nucleotide binding properties of bovine brain uncoating ATPase. Journal of Biological Chemistry. ,vol. 268, pp. 8507- 8513 ,(1993) , 10.1016/S0021-9258(18)52904-5
S L Schmid, J E Rothman, Enzymatic dissociation of clathrin cages in a two-stage process. Journal of Biological Chemistry. ,vol. 260, pp. 10044- 10049 ,(1985) , 10.1016/S0021-9258(17)39209-8
K. Prasad, J. Heuser, E. Eisenberg, L. Greene, Complex formation between clathrin and uncoating ATPase. Journal of Biological Chemistry. ,vol. 269, pp. 6931- 6939 ,(1994) , 10.1016/S0021-9258(17)37464-1
L E Greene, E Eisenberg, Dissociation of clathrin from coated vesicles by the uncoating ATPase. Journal of Biological Chemistry. ,vol. 265, pp. 6682- 6687 ,(1990) , 10.1016/S0021-9258(19)39204-X
H. Schröder, T. Langer, F.U. Hartl, B. Bukau, DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage. The EMBO Journal. ,vol. 12, pp. 4137- 4144 ,(1993) , 10.1002/J.1460-2075.1993.TB06097.X
D.M. Cyr, X Lu, M.G. Douglas, Regulation of Hsp70 function by a eukaryotic DnaJ homolog. Journal of Biological Chemistry. ,vol. 267, pp. 20927- 20931 ,(1992) , 10.1016/S0021-9258(19)36777-8
S P Chock, E Eisenberg, The mechanism of the skeletal muscle myosin ATPase. I. Identity of the myosin active sites. Journal of Biological Chemistry. ,vol. 254, pp. 3229- 3235 ,(1979) , 10.1016/S0021-9258(18)50748-1
R Lindner, E Ungewickell, Clathrin-associated proteins of bovine brain coated vesicles. An analysis of their number and assembly-promoting activity. Journal of Biological Chemistry. ,vol. 267, pp. 16567- 16573 ,(1992) , 10.1016/S0021-9258(18)42040-6
S Schröder, E Ungewickell, Subunit interaction and function of clathrin-coated vesicle adaptors from the Golgi and the plasma membrane. Journal of Biological Chemistry. ,vol. 266, pp. 7910- 7918 ,(1991) , 10.1016/S0021-9258(20)89536-2
K Prasad, W Barouch, L Greene, E Eisenberg, A protein cofactor is required for uncoating of clathrin baskets by uncoating ATPase. Journal of Biological Chemistry. ,vol. 268, pp. 23758- 23761 ,(1993) , 10.1016/S0021-9258(20)80445-1