In vivo real-time measurement of nitric oxide in anesthetized rat brain.

摘要: During the last two decades nitric oxide (.NO) gas has emerged as a novel and ubiquitous intercellular modulator of cell functions. In brain, .NO is implicated in mechanisms synaptic plasticity but it also involved death pathways underlying several neurological diseases. Because its hydrophobicity, small size, rapid diffusion properties, rate pattern concentration changes are critical determinants for understanding diverse actions brain. measurement vivo been challenging task due to low concentration, short half-life, high reactivity with other biological molecules, such superoxide radical, thiols, heme proteins. Electrochemical methods versatile approaches detecting monitoring various neurotransmitters. When associated microelectrodes inserted into brain they provide temporal spatial resolution, allowing measurements neurochemicals physiological environments real-time fashion. To date, electrochemical detection only available technique that provides sensitivity, limit, selectivity, fast response measure dynamics vivo. We have used carbon fiber coated layers Nafion o-phenylenediamine monitor change rat The analytical performance was assessed terms selectivity ratios against major interferents: ascorbate, dopamine, noradrenaline, serotonin, nitrite. For recording experiments, we microelectrode/micropipette array using stereotaxic frame. characterization signals by pharmacological verification. Results support our experimental conditions measured oxidation current reflects variations extracellular space. report results from recordings hippocampus striatum upon stimulation N-methyl-d-aspartate-subtype glutamate receptors. Moreover, kinetics disappearance following pressure ejection solution addressed.