Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

摘要: Systematic manipulations of the culture protocol leading to somatic embryogenesis in alfalfa petiole-derived callus were performed study competence phase during alfalfa. These demonstrated a requirement for acquisition prior induction pathway. Induction was triggered by number synthetic auxins including 2,4-dichlorophenoxyacetic acid (2,4-d). Competence could be acquired presence these as well phenylacetic (PAA), an auxin that did not induce embryogenesis. Different degrees apparently exposure 2,4-d and PAA. Some degree absence treatment. The current understanding concept is discussed.