Improved purification and sulfhydryl analysis of thiosulfate reductase
作者： Thomas R. Chauncey , John Westley
DOI: 10.1016/0167-4838(83)90204-2
关键词: Cysteine 、 Stereochemistry 、 Dithionitrobenzoic Acid 、 Sulfurtransferase 、 Iodoacetamide 、 Bond cleavage 、 Amino acid 、 Thiosulfate 、 Chemistry 、 Glutathione 、 Biochemistry
摘要: Thiosulfate reductase purified 900-fold from an extract of baker's yeast by a new procedure consisted three charge-isomeric species, all with catalytic activity. Amino acid analysis thiosulfate and titrations 5,5'-dithiobis(2-nitrobenzoate) iodo[14C]acetate indicated only one cysteine residue per enzyme molecule. Alkylation this either iodoacetate or iodoacetamide did not inactivate the enzyme, indicating that sulfur-sulfur bond cleavage in substrate does require enzymic sulfhydryl group as attacking nucleophile.
elsevier.com
sci-hub.se 参考文章(24)
Vincent Massey, Charles H. Williams, On the reaction mechanism of yeast glutathione reductase. Journal of Biological Chemistry. ,vol. 240, pp. 4470- 4480 ,(1965) , 10.1016/S0021-9258(18)97085-7
H.D. Peck, E. Fisher, The oxidation of thiosulfate and phosphorylation in extracts of Thiobacillus thioparus. Journal of Biological Chemistry. ,vol. 237, pp. 190- 197 ,(1962) , 10.1016/S0021-9258(18)81384-9
Akira Kaji, W. D. McElroy, MECHANISM OF HYDROGEN SULFIDE FORMATION FROM THIOSULFATE1 Journal of Bacteriology. ,vol. 77, pp. 630- 637 ,(1959) , 10.1128/JB.77.5.630-637.1959
Stephen Zamenhof, [103] Preparation and assay of deoxyribonucleic acid from animal tissue Methods in Enzymology. ,vol. 3, pp. 696- 704 ,(1957) , 10.1016/S0076-6879(57)03446-1
J Westley, L Weng, R L Heinrikson, Active site cysteinyl and arginyl residues of rhodanese. A novel formation of disulfide bonds in the active site promoted by phenylglyoxal. Journal of Biological Chemistry. ,vol. 253, pp. 8109- 8119 ,(1978) , 10.1016/S0021-9258(17)34369-7
Y.S. Im, P.K. Chiang, G.L. Cantoni, Guanidoacetate methyltransferase. Purification and molecular properties. Journal of Biological Chemistry. ,vol. 254, pp. 11047- 11050 ,(1979) , 10.1016/S0021-9258(19)86628-0
Allan G. Gornall, Charles J. Bardawill, Maxima M. David, Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry. ,vol. 177, pp. 751- 766 ,(1949) , 10.1016/S0021-9258(18)57021-6
S-F Wang, M Volini, Studies on the Active Site of Rhodanese Journal of Biological Chemistry. ,vol. 243, pp. 5465- 5470 ,(1968) , 10.1016/S0021-9258(18)91969-1
V P Pigiet, R R Conley, Purification of thioredoxin, thioredoxin reductase, and glutathione reductase by affinity chromatography. Journal of Biological Chemistry. ,vol. 252, pp. 6367- 6372 ,(1977) , 10.1016/S0021-9258(17)39966-0
Torvard C. Laurent, E. Colleen Moore, Peter Reichard, ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES. IV. ISOLATION AND CHARACTERIZATION OF THIOREDOXIN, THE HYDROGEN DONOR FROM ESCHERICHIA COLI B. Journal of Biological Chemistry. ,vol. 239, pp. 3436- 3444 ,(1964) , 10.1016/S0021-9258(18)97742-2