Physiological analysis of mutants of Saccharomyces cerevisiae impaired in sulphate assimilation.

摘要: The assimilation of sulphate in Saccharomyces cerevisiae, comprising the reduction to sulphide and incorporation sulphur atom into a four-carbon chain, requires integrity 13 different genes. To date, functions nine these genes are still not clearly established. A set strains, each bearing mutation one MET gene, was studied. Phenotypic studies enzyme determinations showed that products at least five needed for synthesis an enzymically active sulphite reductase. These MET1, MET5, MET8, MET10 MET20. Wild-type strains S. cerevisiae can use organic metabolites such as homocysteine, cysteine, methionine S-adenosylmethionine sources. They also able inorganic sources sulphate, sulphite, or thiosulphate. Here we show both two atoms thiosulphate used by cerevisiae. Thiosulphate is cleaved prior utilization pathway, metabolism from presence