Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae.
作者： Yolande Surdin-Kerjan , Vladimir Benes , Wilhelm Ansorge , Dominique Thomas , Hélène Cherest
DOI: 10.1093/GENETICS/145.3.627
关键词:
摘要: Strains resistant to the toxic analogues of sulfate, selenate and chromate have been isolated. Their genetic analysis allowed us identify four genes. One, called MET28, encodes a transcriptional factor. The three other genes, SUL1, SUL2 SUL3, encode proteins involved in sulfate transport. sequence Sul1p Sul2p indicate that they are integral membrane exhibiting, respectively, 11 10 transmembrane domains. Moreover, share high degree similarity. Sulfate transport kinetic studies made with parental mutant strains show that, as expected from results, Saccharomyces cerevisiae has two affinity systems. Sul3p shown be regulation gene.
genetics.org参考文章(23)
H.U. Gläser, D. Thomas, R. Gaxiola, F. Montrichard, Y. Surdin-Kerjan, R. Serrano, Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. The EMBO Journal. ,vol. 12, pp. 3105- 3110 ,(1993) , 10.1002/J.1460-2075.1993.TB05979.X
Y Surdin-Kerjan, R Barbey, D Thomas, Gene-enzyme relationship in the sulfate assimilation pathway of Saccharomyces cerevisiae. Study of the 3'-phosphoadenylylsulfate reductase structural gene. Journal of Biological Chemistry. ,vol. 265, pp. 15518- 15524 ,(1990) , 10.1016/S0021-9258(18)55427-2
M. Bissig, B. Hagenbuch, B. Stieger, T. Koller, P.J. Meier, Functional expression cloning of the canalicular sulfate transport system of rat hepatocytes. Journal of Biological Chemistry. ,vol. 269, pp. 3017- 3021 ,(1994) , 10.1016/S0021-9258(17)42040-0
Rodney J. Rothstein, One-step gene disruption in yeast Methods in Enzymology. ,vol. 101, pp. 202- 211 ,(1983) , 10.1016/0076-6879(83)01015-0
A Sirko, M Hryniewicz, D Hulanicka, A Böck, Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster. Journal of Bacteriology. ,vol. 172, pp. 3351- 3357 ,(1990) , 10.1128/JB.172.6.3351-3357.1990
James S. Ketter, Gabor Jarai, Ying Hui Fu, George A. Marzluf, Nucleotide sequence, messenger RNA stability, and DNA recognition elements of cys-14, the structural gene for sulfate permease II in Neurospora crassa. Biochemistry. ,vol. 30, pp. 1780- 1787 ,(1991) , 10.1021/BI00221A008
D. Thomas, R. Barbey, D. Henry, Y. Surdin-Kerjan, Physiological analysis of mutants of Saccharomyces cerevisiae impaired in sulphate assimilation. Microbiology. ,vol. 138, pp. 2021- 2028 ,(1992) , 10.1099/00221287-138-10-2021
John Cram, Characteristics of Sulfate Transport Across Plasmalemma and Tonoplast of Carrot Root Cells Plant Physiology. ,vol. 72, pp. 204- 211 ,(1983) , 10.1104/PP.72.1.204
L. S. Green, D. E. Laudenbach, A. R. Grossman, A region of a cyanobacterial genome required for sulfate transport. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 1949- 1953 ,(1989) , 10.1073/PNAS.86.6.1949
Ivan K. Smith, Characterization of Sulfate Transport in Cultured Tobacco Cells Plant Physiology. ,vol. 58, pp. 358- 362 ,(1976) , 10.1104/PP.58.3.358