Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1.

摘要: Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal tissue. Higher expression of CRISP-3 has been linked poor prognosis and hence it thought act a prognostic marker for cancer. It proposed have role innate immunity but its still unknown. In order understand function, was stably knocked down LNCaP cells. knockdown did not affect cell viability resulted reduced invasiveness. Global gene changes upon were identified by microarray analysis. Microarray data quantitatively validated evaluating seven candidate genes three independent stable clones. Functional annotation differentially expressed adhesion, motility, ion transport be affected among other biological processes. Prostate-specific antigen (PSA, also known Kallikrein 3) top most downregulated whose at level. Interestingly, Annexin A1 (ANXA1), anti-inflammatory protein, knockdown. Re-introduction into clone reversed effect on invasiveness led increased PSA expression. These results suggest that overexpression tumor may maintain higher lower ANXA1 Our indicate associated with could due invasion.