Establishment method of carrier-free chloramphenicol aptamer signal amplifying sensor

摘要: The invention relates to an establishment method of a carrier-free chloramphenicol aptamer signal amplifying sensor, and belongs the field detection substances antibiotics like. aims establish sensor free from carrier, so that experiment cost is reduced as much possible, quick high-sensitivity remaining realized. A used example, through auxiliary short strand which two ends are respectively modified with quenchers fluorophores form complementary dual strand. nucleic acid excision enzyme I(EXO I) added, release circulation amplification fluorescence signals realized, signalamplifying established. Through endonuclease activity EXO I enzyme, in aptamer-target compound obtained by competition affinity can be decomposed target, target material enters cycle reaction obtain amplified signals.