Differential gene expression as an indicator of nitrogen sufficiency in field-grown potato plants

作者: Bernie J. Zebarth , Helen Tai , Sainan Luo , Pete Millard , David De Koeyer

DOI: 10.1007/S11104-011-0793-Z

关键词: AgronomyNitrate reductaseFertilizerBiologyAmmoniumGene expressionNitrogenPlant physiologyCultivarDNS root zone

摘要: Use of an in-season measure crop N sufficiency to guide fertilizer management is one approach match the supply demand. This study examined use gene expression in leaf tissue field-grown potatoes for assessment potato sufficiency. Potato cultivar ‘Shepody’ was grown with six rates (0–250 kg N ha–1). Leaf disks were collected weekly quantification uptake/transport, assimilation, and amino acid metabolism genes by nCounter. Many evaluated responsive supply, but response varied widely among sampling dates. The exception ammonium transporter (AT1) which highly expressed, relatively consistent across dates, closely related root zone soil nitrate concentration negatively correlated total tuber yield. level AT1 as good or better than conventional chemical optical measures current study.

参考文章(53)
J.N. Pearson, J. Finnemann, J.K. Schjoerring, Regulation of the high-affinity ammonium transporter (BnAMT1;2) in the leaves of Brassica napus by nitrogen status. Plant Molecular Biology. ,vol. 49, pp. 483- 490 ,(2002) , 10.1023/A:1015549115471
J. P. Goffart, M. Olivier, D. K. L. MacKerron, A. J. Haverkort, Management of N fertilization of the potato crop using total N-advice software and in-season chlorophyll-meter measurements. decision support systems. pp. 69- 83 ,(2004)
Anna Amtmann, John P. Hammond, Patrick Armengaud, Philip J. White, Nutrient Sensing and Signalling in Plants: Potassium and Phosphorus Advances in Botanical Research. ,vol. 43, pp. 209- 257 ,(2005) , 10.1016/S0065-2296(05)43005-0
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
B. Marshall, Decision Support Systems in Potato Production Potato Biology and Biotechnology#R##N#Advances and Perspectives. pp. 777- 800 ,(2007) , 10.1016/B978-044451018-1/50078-6
B. J. Zebarth, C. J. Rosen, Research perspective on nitrogen BMP development for potato American Journal of Potato Research. ,vol. 84, pp. 3- 18 ,(2007) , 10.1007/BF02986294
Samuel P. Hazen, Yajun Wu, Joel A. Kreps, Gene expression profiling of plant responses to abiotic stress. Functional & Integrative Genomics. ,vol. 3, pp. 105- 111 ,(2003) , 10.1007/S10142-003-0088-4
Hans-Werner Olfs, Klaus Blankenau, Frank Brentrup, Jörg Jasper, Axel Link, Joachim Lammel, Soil- and plant-based nitrogen-fertilizer recommendations in arable farming Journal of Plant Nutrition and Soil Science. ,vol. 168, pp. 414- 431 ,(2005) , 10.1002/JPLN.200520526
Chris M. Long, Sieg S. Snapp, Dave S. Douches, Richard W. Chase, Tuber Yield, Storability, and Quality of Michigan Cultivars in Response to Nitrogen Management and Seedpiece Spacing American Journal of Potato Research. ,vol. 81, pp. 347- 357 ,(2004) , 10.1007/BF02870181