How to overcome genotyping errors in non-invasive genetic mark-recapture population size estimation-A review of available methods illustrated by a case study

摘要: The main goal of non-invasive genetic capture-mark-recapture (CMR) analysis is to gain an unbiased and reliable population size estimate species that cannot be sampled directly. method has becomean importantandwidelyusedtoolto research andmanage wildlifepopulations.However,researchers have struggle with low amplification success rates genotyping errors, which substantially bias subsequent analysis. To receive results minimize the time costs required for microsatellite genotyping, one must carefully choose a species-specific sampling design, methods maximize amount template DNA, could overcome especially when using low-quality samples.This articlereviewsthe literature theprosandcons ofthemain used along process described above. review strengthened by case study on Eurasian otters (Lutra lutra) feces; we tested several their appropriateness accommodate errors. Based this testing, demonstrated high error are key problem in leading severely flawed dataset if no consensus genotype formed. However, even generating genotypes minimizes errors dramatically, show it may not achieve definite eradication all overestimated sizes conventional estimators used. In conjunction these findings, offer step-by-step protocol CMR studies presence rates. 2013 Wildlife Society.