Maternal dazap2 regulates germ granules by counteracting Dynein in zebrafish primordial germ cells
作者： Meredyth M. Forbes , Sophie Rothhämel , Andreas Jenny , Florence L. Marlow
DOI: 10.1016/J.CELREP.2015.06.010
关键词: Dynein 、 Embryonic stem cell 、 Embryo 、 Genetics 、 Germ plasm 、 Oocyte 、 Germ cell 、 Cell biology 、 Germline 、 Biology 、 Zebrafish
摘要: Primordial germ cells (PGCs) are the stem of germline. Generally, germline induction occurs via zygotic factors or inheritance maternal determinants called plasm (GP). GP is packaged into ribonucleoprotein complexes within oocytes and later promotes fate in embryos. Once PGCs specified by either mechanism, components localize to perinuclear granular-like structures. Although zebrafish PGC granules have been studied, regulating their assembly contribution cell development unknown. Here, we show that scaffold protein Dazap2 binds Bucky ball, an essential regulator oocyte polarity assembly, colocalizes with PGCs. Mutational analysis revealed a requirement for (MDazap2) germ-granule maintenance. Through molecular epistasis analyses, MDazap2 epistatic Tdrd7 maintains embryonic counteracting Dynein activity.
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