Histopathological and behavioral assessment of toxin-produced cerebellar lesion: a potent model for cell transplantation studies in the cerebellum.
作者： Aliakbar Haddad-Mashadrizeh , Mohammad Amin Edalatmanesh , Marzieh Moghadas , Mohammad Reza Hashemzadeh , Haniyeh Nikfarjam
DOI:
关键词: Cerebellar hemisphere 、 Dysmetria 、 Analysis of variance 、 Morris water navigation task 、 Pathology 、 Quinolinic acid 、 Purkinje cell 、 Motor learning 、 Biology 、 Cerebellum
摘要: OBJECTIVE The cerebellum is a key structure involved in coordinated motor planning, cognition, learning and memory functions. This study presents permanent model of toxin produced cerebellar lesion characterized according to contemporary cognitive abnormalities. MATERIALS AND METHODS In this experimental study, slow administration quinolinic acid (QA, 5 µl 200 µmol, 1 µl/minute) the right hemisphere (lobule VI) caused noticeable disturbances along with cellular degeneration all treated animals. We assessed behavioral histopathological studies over ten weeks after QA treatment. data were analyzed ANOVA student's t test. RESULTS group showed marked deficits on rotating rod test (p=0.0001), locomotor asymmetry cylinder dysmetria beam balance abnormalities neuromuscular strength hang wire spatial Morris water maze (MWM, p=0.001) fear conditioned passive avoidance (p=0.01) ten-week period compared control Histopathological analysis loss Purkinje cells (p=0.001) granular cell density (p=0.0001) lesioned cerebellum. CONCLUSION Results present show that can remove numerous which respond hemispheric lobule VI thus provide potential for functional cell-based studies.
oaji.net 参考文章(48)
Jr Alvin V. Terry, Spatial navigation (water maze) tasks CRC Press/Taylor & Francis. pp. 153- 166 ,(2009)
Miguel Viana-Baptista, Paulo Bugalho, Bernardo Correa, [Role of the cerebellum in cognitive and behavioural control: scientific basis and investigation models]. Acta Médica Portuguesa. ,vol. 19, pp. 257- 267 ,(2006) , 10.20344/AMP.924
Jeremy D. Schmahmann, Deepak N. Pandya, The Cerebrocerebellar System International Review of Neurobiology. ,vol. 41, pp. 31- 60 ,(1997) , 10.1016/S0074-7742(08)60346-3
Roberta M. Kelly, Peter L. Strick, CEREBELLAR LOOPS WITH MOTOR CORTEX AND PREFRONTAL CORTEX OF A NONHUMAN PRIMATE The Journal of Neuroscience. ,vol. 23, pp. 8432- 8444 ,(2003) , 10.1523/JNEUROSCI.23-23-08432.2003
T W Stone, Neuropharmacology of quinolinic and kynurenic acids. Pharmacological Reviews. ,vol. 45, pp. 309- 379 ,(1993)
Peyman Yeganeh-Doost, Oliver Gruber, Peter Falkai, Andrea Schmitt, The role of the cerebellum in schizophrenia: from cognition to molecular pathways Clinics. ,vol. 66, pp. 71- 77 ,(2011) , 10.1590/S1807-59322011001300009
Hai-xia Lu, Hannah Levis, Nabil Melhem, Terry Parker, Toxin-produced Purkinje cell death: A model for neural stem cell transplantation studies Brain Research. ,vol. 1207, pp. 207- 213 ,(2008) , 10.1016/J.BRAINRES.2008.02.034
F. Middleton, P. Strick, Anatomical evidence for cerebellar and basal ganglia involvement in higher cognitive function Science. ,vol. 266, pp. 458- 461 ,(1994) , 10.1126/SCIENCE.7939688
C. Piochon, T. Irinopoulou, D. Brusciano, Y. Bailly, J. Mariani, C. Levenes, NMDA receptor contribution to the climbing fiber response in the adult mouse Purkinje cell. The Journal of Neuroscience. ,vol. 27, pp. 10797- 10809 ,(2007) , 10.1523/JNEUROSCI.2422-07.2007
C STOODLEY, J SCHMAHMANN, FUNCTIONAL TOPOGRAPHY IN THE HUMAN CEREBELLUM: A META-ANALYSIS OF NEUROIMAGING STUDIES NeuroImage. ,vol. 44, pp. 489- 501 ,(2009) , 10.1016/J.NEUROIMAGE.2008.08.039