Toxin-produced Purkinje cell death: A model for neural stem cell transplantation studies
作者： Hai-xia Lu , Hannah Levis , Nabil Melhem , Terry Parker
DOI: 10.1016/J.BRAINRES.2008.02.034
关键词: Transplantation 、 Penitrem A 、 Neural stem cell 、 Biology 、 Pathology 、 Cerebellum 、 Stem cell 、 Purkinje cell 、 Cerebellar vermis 、 Calbindin 、 Neuroscience 、 Clinical neurology 、 Developmental biology 、 General Neuroscience 、 Molecular biology
摘要: Purkinje cell loss is the hallmark of cerebellar ataxias. Here fungal neurotoxin Penitrem A was used to create partially Purkinje-cell-deficient cerebella in neonate and young adult rats suitable for use neural stem transplantation studies. I.p. administration P3, P6 11-week old caused noticeable tremor all treated animals that lasted between 1 3 days more immediate rat pups than rats. Quantification cresyl violet stained sections showed cells were preferentially lost vermis specifically folia VI IX (P<0.001-0.05). No change occurred number I-III folium X. These results confirmed by calbindin binding layer appearance enlarged vacuolated mitochondria. The present study show can remove immature cerebellum thus provide a potential model micro-environmental cues vivo differentiation from transplanted and/or intrinsic cells.
elsevier.com
sci-hub.se 参考文章(36)
K S Bedi, L F Campbell, T M Mayhew, A fractionator study of the effects of undernutrition during early life on rat Purkinje cell numbers (with a caveat on the use of nucleoli as counting units). Journal of Anatomy. ,vol. 181, pp. 199- 208 ,(1992)
John Dobbing, The development of the blood-brain barrier. Progress in Brain Research. ,vol. 29, pp. 417- 427 ,(1968) , 10.1016/S0079-6123(08)64172-2
Joseph Altman, Shirley A. Bayer, Development of the Cerebellar System: In Relation to Its Evolution, Structure, and Functions ,(1996)
Barbara Carletti, Piercesare Grimaldi, Lorenzo Magrassi, Ferdinando Rossi, Specification of cerebellar progenitors after heterotopic-heterochronic transplantation to the embryonic CNS in vivo and in vitro. The Journal of Neuroscience. ,vol. 22, pp. 7132- 7146 ,(2002) , 10.1523/JNEUROSCI.22-16-07132.2002
Isabelle Dusart, Matti S. Airaksinen, Constantino Sotelo, Purkinje Cell Survival and Axonal Regeneration Are Age Dependent: AnIn VitroStudy The Journal of Neuroscience. ,vol. 17, pp. 3710- 3726 ,(1997) , 10.1523/JNEUROSCI.17-10-03710.1997
Andrea Lupi, Giannettore Bertagnoni, Matteo Salgarello, Piergiuseppe Orsolon, Veronica Malfatti, Pierluigi Zanco, Cerebellar vermis relative hypermetabolism: an almost constant PET finding in an injured brain. Clinical Nuclear Medicine. ,vol. 32, pp. 445- 451 ,(2007) , 10.1097/RLU.0B013E3180537621
B.J. Wilson, T. Hoekman, W.-D. Dettbarn, Effects of a fungus tremorgenic toxin (penitrem A) on transmission in rat phrenic nerve-diaphragm preparations Brain Research. ,vol. 40, pp. 540- 544 ,(1972) , 10.1016/0006-8993(72)90159-X
Virginie Sottile, Meng Li, Paul J. Scotting, Stem cell marker expression in the Bergmann glia population of the adult mouse brain. Brain Research. ,vol. 1099, pp. 8- 17 ,(2006) , 10.1016/J.BRAINRES.2006.04.127
Xue-Zhi Sun, Yoshi-Nobu Harada, Rui Zhang, Chun Cui, Sentaro Takahashi, Yoshihiro Fukui, A genetic mouse model carrying the nonfunctional xeroderma pigmentosum group G gene. Congenital Anomalies. ,vol. 43, pp. 133- 139 ,(2003) , 10.1111/J.1741-4520.2003.TB01037.X
C. Sotelo, R. M. Alvarado-Mallart, R. Gardette, F. Crepel, Fate of grafted embryonic Purkinje cells in the cerebellum of the adult "Purkinje cell degeneration" mutant mouse. I. Development of reciprocal graft-host interactions. The Journal of Comparative Neurology. ,vol. 295, pp. 165- 187 ,(1990) , 10.1002/CNE.902950202