Curcumin alters gene expression-associated DNA damage, cell cycle, cell survival and cell migration and invasion in NCI-H460 human lung cancer cells in vitro.

摘要: Lung cancer is the most common cause of mortality and new cases are on increase worldwide. However, treatment lung remains unsatisfactory. Curcumin has been shown to induce cell death in many human cells, including cells. effects curcumin genetic mechanisms associated with these actions remain unclear. (2 µM) was added NCI-H460 cells were incubated for 24 h. Total RNA extracted from isolated cDNA synthesis, labeling, microarray hybridization flour‑labeled hybridized chip. Localized concentrations fluorescent molecules detected quantified using Expression Console software (Affymetrix) default RMA parameters. GeneGo used key genes involved their possible interaction pathways. The results showed that ~170 significantly upregulated 577 downregulated curcumin‑treated Specifically, up‑ included CCNE2, DNA damage; ID3, survival 146 a >2- 3-fold change TP53INP1 gene, CDC6, CDCA5, TAKMIP2, CDK14, CDK5, CDCA76, CDC25A, CDC5L SKP2, cycle; CARD6, ID1 ID2 genes, BRMS1L, migration invasion. Additionally, 59 exhibited >4-fold change, DDIT3 while 97 had >3- 4-fold DDIT4 CCPG1 cycle 321 GADD45A CGREF1 cycle, TNFRSF10B, GAS5, TSSC1 TNFRSF11B ARHAP29 CADM2 In conclusion, gene alterations provide information regarding cytotoxic mechanism at level additional biomarkers or targets cancer.