Down-regulation of lncRNA, GAS5 decreases chemotherapeutic effect of dendrosomal curcumin (DNC) in breast cancer cells
作者： Mohammad Javad Dehghan Esmatabadi , Maryam Motamedrad , Majid Sadeghizadeh
DOI: 10.1016/J.PHYMED.2018.03.022
关键词:
摘要: Abstract Background Despite positive results obtained from anticancer activities of curcumin, there are some obstacles that limit its use as an agent. Hypothesis/purpose Different methods such employing the dendrosomal curcumin (DNC) were examined to overcome problems. There is increasing evidence representing long non-coding RNAs play important roles in biological processes. In this study, we focused on GAS5 anti-cancer effects DNC breast cancer. Methods We used several including MTT assay, apoptosis cell cycle analysis, transwell migration assay and RT-PCR. Results observed a significant increase expression Tusc7, genes with treatment MCF7, MDA-MB231, SKBR3 cells. Also, combination down-regulation showed lower percentages apoptotic cells higher level penetration through membrane compared alone. Furthermore, induced number sub G1/G1 phase decrease G2/M cycle. But, after alone opposite was DNC. Conclusion can suppress many aspects cancer cells, it seems co-treatment over-expression may provide clinically useful tool for drug-resistance
sci-hub.se 参考文章(60)
Amy N. Shore, Jason I. Herschkowitz, Jeffrey M. Rosen, Noncoding RNAs involved in mammary gland development and tumorigenesis: there's a long way to go. Journal of Mammary Gland Biology and Neoplasia. ,vol. 17, pp. 43- 58 ,(2012) , 10.1007/S10911-012-9247-3
Dandan Yin, Xuezhi He, Erbao Zhang, Rong Kong, Wei De, Zhihong Zhang, Long noncoding RNA GAS5 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer Medical Oncology. ,vol. 31, pp. 253- ,(2014) , 10.1007/S12032-014-0253-8
Ning Liang, Yong Wang, Chaozhao Liang, Xin Li, Fei Sun, Jie Lian, Xiansheng Zhang, Hui Tian, Altered microRNA expression in patients with non-obstructive azoospermia. Reproductive Biology and Endocrinology. ,vol. 7, pp. 13- 13 ,(2009) , 10.1186/1477-7827-7-13
J. L. Sebaugh, Guidelines for accurate EC50/IC50 estimation Pharmaceutical Statistics. ,vol. 10, pp. 128- 134 ,(2011) , 10.1002/PST.426
Mao Li, Zhuo Zhang, Donald L. Hill, Hui Wang, Ruiwen Zhang, Curcumin, a dietary component, has anticancer, chemosensitization, and radiosensitization effects by down-regulating the MDM2 oncogene through the PI3K/mTOR/ETS2 pathway. Cancer Research. ,vol. 67, pp. 1988- 1996 ,(2007) , 10.1158/0008-5472.CAN-06-3066
M Mourtada-Maarabouni, M R Pickard, V L Hedge, F Farzaneh, G T Williams, GAS5, a non-protein-coding RNA, controls apoptosis and is downregulated in breast cancer. Oncogene. ,vol. 28, pp. 195- 208 ,(2009) , 10.1038/ONC.2008.373
Mohammad N Sarbolouki, Majid Sadeghizadeh, Mohammad M Yaghoobi, Ali Karami, Tahmineh Lohrasbi, Dendrosomes: a novel family of vehicles for transfection and therapy Journal of Chemical Technology & Biotechnology. ,vol. 75, pp. 919- 922 ,(2000) , 10.1002/1097-4660(200010)75:10<919::AID-JCTB308>3.0.CO;2-S
M. Mourtada-Maarabouni, V. L. Hedge, L. Kirkham, F. Farzaneh, G. T. Williams, Growth arrest in human T-cells is controlled by the non-coding RNA growth-arrest-specific transcript 5 (GAS5). Journal of Cell Science. ,vol. 121, pp. 939- 946 ,(2008) , 10.1242/JCS.024646
MaLing Gou, Ke Men, HuaShan Shi, MingLi Xiang, Juan Zhang, Jia Song, JianLin Long, Yang Wan, Feng Luo, Xia Zhao, ZhiYong Qian, Curcumin-loaded biodegradable polymeric micelles for colon cancer therapy in vitro and in vivo Nanoscale. ,vol. 3, pp. 1558- 1567 ,(2011) , 10.1039/C0NR00758G
Vahid Erfani-Moghadam, Alireza Nomani, Mina Zamani, Yaghoub Yazdani, Farhood Najafi, Majid Sadeghizadeh, A novel diblock copolymer of (monomethoxy poly [ethylene glycol]-oleate) with a small hydrophobic fraction to make stable micelles/polymersomes for curcumin delivery to cancer cells International Journal of Nanomedicine. ,vol. 9, pp. 5541- 5554 ,(2014) , 10.2147/IJN.S63762