Uterine Temporal Response to Acute Exposure to 17α-Ethinyl Estradiol in the Immature Rat

摘要: The rat uterus responds to acute estrogen treatment with a series of well-characterized physiological responses; however, the gene expression changes required elicit these responses have not been fully characterized. In order understand early events induced by exposure in vivo, we evaluated temporal immature after single dose 17 alpha-ethinyl estradiol (EE) microarray analysis, evaluating 15,923 genes. Immature 20-day-old rats were exposed EE (10 microg/kg), and effects on uterine histology, weight, determined 1, 2, 8, 24, 48, 72, 96 h. 3867 genes, at least one time point (p < or = 0.0001), 1.5-fold (up- downregulated). Specifically, 116, 3030, 2076, 381, 445, 125 genes was modified h EE, respectively 0.0001, t-test). At tissue organ level, clear uterotrophic response elicited only 8 h, reaching maximum 24 remaining detectable even exposure. phenotypic sequential transcriptional status large number program that involves multiple molecular pathways. Using Gene Ontology better exposure, earliest whose products are involved regulation signal transduction, followed implicated protein synthesis, energy utilization, solute transport, cell proliferation differentiation, remodeling, immunological among other compendium here presented represents comprehensive compilation estrogen-responsive response.