Development of electrochemical genosensor for MYCN oncogene detection using rhodamine B as electroactive label

作者: Thalles Douglas Souza e Silva , Ana Cristina Honorato de Castro , Vinícius de Rezende Rodovalho , João Marcos Madurro , Ana Graci Brito Madurro

DOI: 10.1007/S10008-016-3326-0

关键词: Detection limitBiomarkerElectrochemistryDNAChemistryNeuroblastomaMolecular biologyDNA–DNA hybridizationRhodamine BComplementary DNA

摘要: A novel electrochemical genosensor based on a graphite electrode modified with poly(4-aminophenol) has been constructed for prognostic of neuroblastoma, malignant tumor originating from embryonic precursor cells the sympathetic nervous system and associated amplification MYCN oncogene. The exhibited distinct electrical morphological properties using rhodamine B as indicator DNA hybridization. detection limit was evaluated to be 0.47 μmol L−1 (n = 3), selective complementary DNA, serum sample. This sensing platform successfully applied detect MYCN, an important biomarker neuroblastoma.

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