Non-destructive approach for the characterization of the in situ carotenoid deposition in gac fruit aril

摘要: Abstract Carotenoid deposition in gac fruit aril, carrot root, and tomato was investigated situ using a combination of light microscopy, UV/Vis transmission spectroscopy, diffuse reflectance spectroscopy. Reflectance data transformed to absorbance the Kubelka-Munk function. As shown by carotenoids aril were deposited round-shaped chromoplasts, whereas characterized needle-shaped, crystalloid chromoplasts. Crystalline carotenoid could be confirmed via polarized microscopy. By identical means, crystalline lycopene β-carotene revealed, most likely form microcrystalline aggregates. This hypothesis supported simple solubility estimation based on lipid contents aril. comparison absorption spectra recorded from aggregated genuine extracts, authentic standards, spectra, an H-aggregated tomato, J-aggregated forms carrot, lycopene, β-carotene, possibly lipid-dissolved confirmed. The present approach used gain insights into aggregate planta , representing low-cost, non-destructive -characterization method.