Comparison of lipid content, surface membrane fluidity, and temperature dependence of cis-diamminedichloroplatinum(II) accumulation in sensitive and resistant human ovarian carcinoma cells.

摘要: We conducted studies to determine whether the cisplatin (DDP)-resistant human ovarian carcinoma cell line designated 2008/DDP has membrane changes which could slow passive diffusion of drug into cells, thereby accounting for observed reduction in DDP accumulation cells compared sensitive parental 2008 cells. To this end, we three indicators lipid composition and fluidity resistant In a comparison major classes, had 17% increase phosphatidyl choline, an 11% ethanolamine relative line. There were no differences other phospholipids or free cholesterol content lines. Secondly, results fluorescence polarization measurements on whole each using plasma specific probe trimethylammonium diphenylhexatriene revealed difference between lines at both 28 degrees C 37 C. Finally, examination temperature-dependence over 1 h produced Arrhenius plots similar shape Both linear from 40 down break point 4 12 The Q10's 30 greater than 2 not significantly different. Results these 3 comparisons substantial similarity bulk two conclude that decreased our is due would be expected retard