A reference genome of the European beech (Fagus sylvatica L.).

摘要: Background The European beech is arguably the most important climax broad-leaved tree species in Central Europe, widely planted for its valuable wood. Here, we report 542 Mb draft genome sequence of an up to 300-year-old individual (Bhaga) from undisturbed stand Kellerwald-Edersee National Park central Germany. Findings Using a hybrid assembly approach, Illumina reads with short- and long-insert libraries, coupled long Pacific Biosciences reads, obtained assembled size Mb, line flow cytometric estimation. largest scaffold was 1.15 N50 length 145 kb, L50 count 983. contained 0.12% Ns. A Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis retrieved 94% complete BUSCO genes, well range other high-quality genomes trees. total 62,012 protein-coding genes were predicted, assisted by transcriptome sequencing. In addition, are reporting efficient method extracting high-molecular-weight DNA dormant buds, which contamination environmental bacteria fungi kept at minimum. Conclusions will be resource reference future population genomics studies on evolution past climate change adaptation helpful identifying e.g., involved drought tolerance, order select breed individuals adapt forestry Europe. continuously updated browser download page can accessed beechgenome.net, include versions Bhaga, as new sequencing approaches develop.