Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1

摘要: The newly emergent human coronavirus HKU1 (HCoV-HKU1) was first identified in Hong Kong 2005. Infection by HCoV-HKU1 occurs worldwide and causes syndromes such as the common cold, bronchitis, pneumonia. CoV main protease (M(pro)), which is a key enzyme viral replication via proteolytic processing of replicase polyproteins, has been recognized an attractive target for rational drug design. In this study, we report structure M(pro) complex with Michael acceptor, inhibitor N3. provides high-quality model group 2A CoVs, are distinct from 2B CoVs severe acute respiratory syndrome CoV. structure, together activity assays, supports relative conservation at P1 position that discovered sequencing genome. Combined structural data other M(pro)s, reported here insights into both substrate preference design antivirals targeting CoVs.