Fgf10 is essential for maintaining the proliferative capacity of epithelial progenitor cells during early pancreatic organogenesis

摘要: The importance of mesenchymal-epithelial interactions for the proper development pancreas has been acknowledged since early 1960s, even though molecule(s) mediating this process have remained unknown. We demonstrate here that Fgf10, a member fibroblast growth factor family (FGFs), plays an essential role in process. show Fgf10 is expressed mesenchyme directly adjacent to dorsal and ventral pancreatic epithelial buds. In Fgf10–/– mouse embryos, evagination epithelium initial formation buds appear normal. However, subsequent growth, differentiation branching morphogenesis are arrested; primarily due dramatic reduction proliferation progenitor cells marked by production homeobox protein PDX1. Furthermore, FGF10 restores population PDX1-positive organ cultures derived from embryos. These results indicate signalling required normal should prove useful devising methods expand cells.