Estrous stage- and animal age-independent superovulation in the BrlHan:WIST@Jcl(GALAS) rat.
作者： Yusuke SOTOMARU , Tsutomu KAMISAKO , Kyoji HIOKI
关键词: Age differences 、 Biology 、 Embryo transfer 、 Estrous cycle 、 Endocrinology 、 Internal medicine
摘要: In most strains of rats, the effects treatment for induction superovulation show major strain differences and are strongly influenced by stage estrous cycle. This study demonstrated, however, that was easily induced in Wistar Brl Han:WIST@Jcl(GALAS) rats PMSG hCG administration. To confirm such treatment, we studied age egg collection efficiency. After intraperitoneal administration 150 IU/kg 75 given 48 h apart, mean numbers oocytes obtained from at 4, 8, 12, 20 28 weeks were 38.9, 33.5, 46.1, 26.9 21.3, respectively. No caused observed. an embryo transfer experiment, fertilized eggs superovulated each week showed equivalent viability until full-term to those untreated rats. These results suggest stage-independent is effective not only pre-pubertal but also adult
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