Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.

摘要: The natural interferon (IFN)-produc- ing cell is now known to be identical the plasma- cytoid dendritic (PDC). These are Lin - , CD123, CD11c and human leukocyte antigen- DR cells that secrete large amounts of IFN- (1-2 IU/cell) when stimulated by enveloped viruses such as herpes simplex virus. In current study, we have evaluated chemokine expression virally PDC. Up-regulation mRNA for CCL4, CCL3, CCL5, CCL2, CXC ligand (CXCL)10 in virus-stimu- lated PDC was detected RNAse protection as- says. contrast, PDC-depleted peripheral blood mononuclear did not up-regulate these species upon viral stimulation. Enzyme-linked im- munosorbent assay and/or intracellular flow cy- tometry confirmed production proteins, studies indicated overlapping other cytokines/chemokines Endocytosis plays a critical role chemo- kine induction, disruption pathway inhib- its response. However, transcription genes required induction. Autocrine signaling could ac- count portion CXCL10 CCL2 but responsible induction kines. To evaluate functional che- mokines, chemotaxis assays were performed using supernatants from Acti- vated T killer cells, nao¨ve preferentially recruited supernatants. Migration subsequently inhibited addition neutralizing antibody CCL4 CXCL10. We hypothesize induced mokine pivotal hom- leukocytes J. Leukoc. Biol. 75: 504-514; 2004.