Epigallocatechin-3-gallate inhibited cancer stem cell-like properties by targeting hsa-mir-485-5p/RXRα in lung cancer.

摘要: Non-small-cell lung cancer (NSCLC) appears to be a significant threat public health worldwide. MicroRNAs have been identified as regulators for the development of NSCLC. Previous reports suggested that hsa-mir-485-5p is dysregulated in various cancers. RXRα, kind nuclear receptor, an effective target treatment. Cancer stem cells (CSCs) are recognized main cause tumor metastasis, recurrence, and chemotherapy resistance. However, mechanism by which RXRα modulate CSCs NSCLC remains unknown. Here, we found was decreased serum samples from patients with cells. Meanwhile, epigallocatechin-3-gallate (EGCG), anticancer compound extracted green tea, can enhance expression. Hsa-mir-485-5p mimics markedly inhibited cell growth induced apoptosis. inhibition significantly enriched CSC-like traits. Moreover, bioinformatics analysis predicted binding correlation between confirmed dual-luciferase reporter assay. We observed increased EGCG could inhibit levels dose dependently. In addition, upregulation or activation expanded properties cells, whereas inactivation exert reverse phenomenon. Consistently, vivo experiments also validated repress characteristics modulating hsa-mir-485-5p/RXRα axis. Our findings may reveal novel molecular treatment