Phenolics Composition and Antioxidant Activity of Sweet Basil (Ocimum basilicum L.)

作者: Chamila Jayasinghe , Naohiro Gotoh , Tomoko Aoki , Shun Wada

DOI: 10.1021/JF034269O

关键词: ChemistryOcimumRosmarinic acidPhenolsOrganic chemistryLiquid chromatography–mass spectrometrySweet BasilBasilicumChromatographyAntioxidantBiphenyl compound

摘要: The antioxidant activity of a methanolic extract Ocimum basilicum L. (sweet basil) was examined using different in vitro assay model systems. crude fractionated on Sephadex LH-20 column, and six fractions were identified. DPPH scavenging system the oxidation soy phosphotidylcholin liposome used to evaluate each fraction. Fraction IV showed strongest followed by V VI. Phenolic compounds responsible for antioxidative characterized atmospheric pressure chemical ionization liquid chromatography-mass spectrometry. major compound fraction confirmed as rosmarinic acid (1)H NMR characteristic fragmentations mass spectrum. Moreover, native examined. results that one can capture 1.52 radicals, furthermore, existence synergistic effect between alpha-tocopherol revealed.

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