[Next Generation Sequencing Technologies (NGST) -- Development and Applications]
作者： Zsuzsanna Mihály , Balázs Győrffy
关键词: Humanities 、 Proteomics methods 、 Biology 、 Genetics
摘要: A kovetkező generacios szekvenalasi technologiak megjelenese az elmult tiz evben jelentős előrelepest jelentett a gyors es hatekony genomialis DNS-szekvenalas teren. Tanulmanyunkban attekintjuk 1975-os sangeri lancterminacios szekvenalastol valos idejű lehetőve valasaig vezető modszertani vivmanyokat. klonalis amplikonokkal dolgozo, sok szalon parhuzamosan futo modszerek alapjai. Manapsag leginkabb funkcionalis genomikai alapkutatasban alkalmazzak ezen technologiakat, amelyek szignaltranszdukcios utvonalak, ontologiak, proteomikai, metabolomikai eredmenyeknek metaanalizise soran nelkulozhetetlen informaciot adnak. Bar klinikumban rutinmodon meg csak elvetve alkalmaznak szekvenatorokat, azonban onkologiaban, kardiologiaban epidemiologiaban mar van igeny technologia altal elerhető extra ismeretekre. Az elterjedes fő gatja adatelemzesi standardizaltsagnak hianya,...
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