Micropropagation of recalcitrant-seeded Ekebergia capensis Sparrm. using in vitro nodal segments and roots
作者： E. Hajari , P. Berjak , N.W. Pammenter , M.P. Watt
DOI: 10.1080/14620316.2009.11512485
关键词: Shoot 、 Vegetative reproduction 、 Germplasm 、 Micropropagation 、 Recalcitrant seed 、 Botany 、 Biology 、 Ekebergia capensis
摘要: SummaryThe present study reports the development of in vitro micropropagation protocols for Ekebergia capensis Sparrm., a species that produces unstorable recalcitrant (non-orthodox) seeds. Suitable were established shoots generated from nodal segments excised vitro-germinated seedlings, and adventitious intact roots. Difficulties experienced when attempts made to micropropagate germplasm saplings. The developed have important applications conservation (cryopreservation clonal multiplication) this species.
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