Recombinant nucleocapsid protein based single serum dilution ELISA for the detection of antibodies to infectious bronchitis virus in poultry
作者： Sunil K. Pradhan , Nitin M. Kamble , Aravind S. Pillai , Satish S. Gaikwad , Sagar A. Khulape
DOI: 10.1016/J.JVIROMET.2014.08.015
关键词: Standard curve 、 Serial dilution 、 Avian infectious bronchitis 、 Recombinant DNA 、 Antibody 、 Antibody titer 、 Biology 、 Infectious bronchitis virus 、 Virology 、 Virus
摘要: Avian infectious bronchitis is ubiquitous and highly contagious disease of poultry, with profound effect on commercial poultry production. For effective control virus (IBV), quick specific diagnosis utmost importance. In this study, the was isolated from clinical samples India full length nucleocapsid (N) gene amplified, cloned expressed in a prokaryotic system. The purified recombinant N protein based single serum dilution enzyme linked immunosorbent assay (ELISA) developed for IBV to measure antibody sera chickens. A total 310 chicken were tested using IDEXX kit along developed. linear correlation obtained between predicted titres at working 1:100 corresponding observed as determined by standard serial method. Regression analysis used construct curve which an equation derived confirmed their correlation. then extrapolate predicated ELISA titer corrected absorbance readings dilution. proved be (95.8%) sensitive (96.8%) when compared test.
elsevier.com
sci-hub.se 参考文章(31)
D.Y. ZHANG, J.Y. ZHOU, J. FANG, J.X. HU JQ WU, A.X. MU, An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli Veterinarni Medicina. ,vol. 50, pp. 336- 344 ,(2018) , 10.17221/5632-VETMED
K. Dhama, T. Bhuvaneshwari, B.B. Dash, S.D. Singh, Biological characterization of Indian isolates of infectious bronchitis virus Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases. ,vol. 30, pp. 11- 14 ,(2009)
Michael M.C. Lai, David Cavanagh, The molecular biology of coronaviruses Advances in Virus Research. ,vol. 48, pp. 1- 100 ,(1997) , 10.1016/S0065-3527(08)60286-9
N.N. Lugovskaya, A.V. Scherbakov, A.S. Yakovleva, M.A. Tsyvanyuk, N.S. Mudrak, V.V. Drygin, A.V. Borisov, Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay Journal of Virological Methods. ,vol. 135, pp. 292- 296 ,(2006) , 10.1016/J.JVIROMET.2006.03.019
Hongying Chen, Bruce Coote, Simon Atree, Julian A. Hiscox, Evaluation of a nucleoprotein-based enzyme-linked immunosorbent assay for the detection of antibodies against infectious bronchitis virus. Avian Pathology. ,vol. 32, pp. 519- 526 ,(2003) , 10.1080/0307945031000154125
Anna K. Williams, Wang Li, Loyd W. Sneed, Ellen W. Collisson, Comparative analyses of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronaviruses. Virus Research. ,vol. 25, pp. 213- 222 ,(1992) , 10.1016/0168-1702(92)90135-V
M. E. G. Boursnell, T. D. K. Brown, I. J. Foulds, P. F. Green, F. M. Tomley, M. M. Binns, Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. Journal of General Virology. ,vol. 68, pp. 57- 77 ,(1987) , 10.1099/0022-1317-68-1-57
Abdul Ndifuna, Anna K. Waters, Minglong Zhou, Ellen W. Collisson, Recombinant nucleocapsid protein is potentially an inexpensive, effective serodiagnostic reagent for infectious bronchitis virus Journal of Virological Methods. ,vol. 70, pp. 37- 44 ,(1998) , 10.1016/S0166-0934(97)00170-5
Niraj Kumar Singh, Sohini Dey, C Madhan Mohan, Jag Mohan Kataria, Vikram N Vakharia, None, Evaluation of four enzyme linked immunosorbent assays for the detection of antibodies to infectious bursal disease in chickens. Journal of Virological Methods. ,vol. 165, pp. 277- 282 ,(2010) , 10.1016/J.JVIROMET.2010.02.008
D. B. Snyder, W. W. Marquardt, E. T. Mallinson, P. K. Savage, D. C. Allen, Rapid Serological Profiling by Enzyme-Linked Immunosorbent Assay. III. Simultaneous Measurements of Antibody Titers to Infectious Bronchitis, Infectious Bursal Disease, and Newcastle Disease Viruses in a Single Serum Dilution Avian Diseases. ,vol. 28, pp. 12- 24 ,(1984) , 10.2307/1590125