Correlation Coefficients Between Different Methods of Expressing Bacterial Quantification Using Real Time PCR

摘要: The applications of conventional culture-dependent assays to quantify bacteria populations are limited by their dependence on the inconsistent success different culture-steps involved. In addition, some can be pathogenic or a source endotoxins and pose health risk researchers. Bacterial quantification based real-time PCR method overcome above-mentioned problems. However, using this approach is commonly expressed as absolute quantities even though composition samples (like those digesta) vary widely; thus, final results may affected if not properly homogenized, especially when multiple pooled together before DNA extraction. objective study was determine correlation coefficients between four methods expressing output data PCR-based bacterial quantification. were: (i) common cell number specific per gram digesta; (ii) Livak Schmittgen, ΔΔCt method; (iii) Pfaffl equation; (iv) simple relative ratio total cells. Because effect population in obtained ΔCt-based (ΔΔCt Pfaffl), these lack acceptable consistency used valid reliable studies. On other hand, because variable compositions digesta samples, corresponding cells same sample more accurate population.