Nano-Mechanical Properties of Heat Inactivated Bacillus anthracis and Bacillus thuringiensis Spores

作者: Jessica L. Poindexter

DOI:

关键词: Bacillus thuringiensisBiophysicsContact modelSurface elasticityHeat inactivationMicrobiologySporeNano-GerminationBiologyBacillus anthracis

摘要: Abstract : B. thuringiensis spores must have similar properties to anthracis be a good simulant in counter-proliferation studies. In particular, they behave way when exposed high temperatures for short periods of time as would caused by an explosion. This research project compares surface elasticities four different spore sample types, spores, heat inactivated and spores. Heat were temperature just enough that no observed germinate. Elasticity values the surfaces determined measuring reflection transmission acoustic waves between atomic force microscope (AFM) tip, assuming Hertz contact model. had higher more variable elasticity then inactivation decrease. Calculated average 3.73 GPa anthracis, 2.73 4.67 thuringiensis, 3.57 thuringiensis.

参考文章(19)
Roy H. Doi, Sporulation and Germination Bacillus. pp. 169- 215 ,(1989) , 10.1007/978-1-4899-3502-1_8
Shanmuga Sozhamannan, Michael D Chute, Farrell D McAfee, Derrick E Fouts, Arya Akmal, Darrell R Galloway, Alfred Mateczun, Leslie W Baillie, Timothy D Read, The Bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages BMC Microbiology. ,vol. 6, pp. 34- 34 ,(2006) , 10.1186/1471-2180-6-34
P M Kutima, P M Foegeding, Involvement of the spore coat in germination of Bacillus cereus T spores. Applied and Environmental Microbiology. ,vol. 53, pp. 47- 52 ,(1987) , 10.1128/AEM.53.1.47-52.1987
Leatrice Y. Santo, Roy H. Doi, Ultrastructural Analysis During Germination and Outgrowth of Bacillus subtilis Spores Journal of Bacteriology. ,vol. 120, pp. 475- 481 ,(1974) , 10.1128/JB.120.1.475-481.1974
Mohd Saif Zaman Mohd. Saif Zaman, Anita Goyal Anita Goyal, GP Dubey, PK Gupta, Harish Chandra Harish Chandra, TK Das, Munia Ganguli Munia Ganguli, Yogendra Singh Yogendra Singh, Imaging and analysis of Bacillus anthracis spore germination Microscopy Research and Technique. ,vol. 66, pp. 307- 311 ,(2005) , 10.1002/JEMT.20174
Elke Y. Wuytack, Chris W. Michiels, A study on the effects of high pressure and heat on Bacillus subtilis spores at low pH. International Journal of Food Microbiology. ,vol. 64, pp. 333- 341 ,(2001) , 10.1016/S0168-1605(00)00478-5
Tatyana G. Kuznetsova, Maria N. Starodubtseva, Nicolai I. Yegorenkov, Sergey A. Chizhik, Renat I. Zhdanov, Atomic force microscopy probing of cell elasticity. Micron. ,vol. 38, pp. 824- 833 ,(2007) , 10.1016/J.MICRON.2007.06.011
Markus Arnoldi, Monika Fritz, Edmund Bäuerlein, Manfred Radmacher, Erich Sackmann, Alexei Boulbitch, Bacterial turgor pressure can be measured by atomic force microscopy Physical Review E. ,vol. 62, pp. 1034- 1044 ,(2000) , 10.1103/PHYSREVE.62.1034
David L Wilson, Kenneth S. Kump, Steven J. Eppell, Roger E. Marchant, Morphological restoration of atomic force microscopy images Langmuir. ,vol. 11, pp. 265- 272 ,(1995) , 10.1021/LA00001A045
Lyndsay Radnedge, Peter G. Agron, Karen K. Hill, Paul J. Jackson, Lawrence O. Ticknor, Paul Keim, Gary L. Andersen, Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Applied and Environmental Microbiology. ,vol. 69, pp. 2755- 2764 ,(2003) , 10.1128/AEM.69.5.2755-2764.2003