Imaging and analysis of Bacillus anthracis spore germination

摘要: External and internal changes occurring during the process of germination Bacillus anthracis spores were observed through atomic force microscopy (AFM) transmission electron (TEM), respectively. AFM studies showed that in response to L-alanine (4 mM), as a germinant, spore germinates into vegetative cell 3 hours. The temporal size gradual but major change was between second third hour. TEM presence varied layers, which is accordance with previous studies. However, integrity these layers lost gradually germination. inner membrane remains intact even until late stages germination, whereas coat, outer membrane, cortical are discarded at second-hour stage. results indicate sequential B. similar other species group.