Synapsin I in PC12 cells. II. Evidence for regulation by NGF of phosphorylation at a novel site

摘要: NGF treatment of PC12 cells caused a rapid increase in the state phosphorylation synapsin I. This I is accompanied by decrease its electrophoretic mobility on SDS-PAGE. Phosphopeptide fingerprint analysis revealed that this occurred particular phosphopeptide, designated peptide N. Phosphoserine was only phosphoamino acid detected Partially purified substrate for several protein kinases known to be capable phosphorylating brain I, but none these phosphorylated The results suggest NGF-stimulated may mediated novel kinase.