Single-Cell Real-Time Visualization and Quantification of Perylene Bioaccumulation in Microorganisms
作者： Xin Jin , Xuejun Guo , Deshu Xu , Yanna Zhao , Xinghui Xia
关键词: Cell 、 Fluorescence microscope 、 Perylene 、 Microorganism 、 Staphylococcus aureus 、 Efflux 、 Biology 、 Escherichia coli 、 Biophysics 、 Bioaccumulation 、 Microbiology
摘要: Bioaccumulation of perylene in Escherichia coli and Staphylococcus aureus was visualized quantified real time with high sensitivity at temporal resolution. For the first time, single-molecule fluorescence microscopy (SMFM) a microfluidic flow chamber temperature control has enabled us to record dynamic process bioaccumulation single bacterial cells examine cell-to-cell heterogeneity. Although identical genomes, individual E. exhibited degree heterogeneity accumulation dynamics, as shown by coefficient variation (C.V = 1.40). This remarkable only live cells. However, dead S. showed similar patterns low 0.36). We found that efflux systems associated Tol C played an essential role coli, which caused significantly lower ...
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