作者: Tadeusz Pacuszka , Peter H. Fishman
DOI: 10.1016/0005-2760(91)90036-H
关键词: Phosphatidylethanolamine 、 Biochemistry 、 Oligosaccharide 、 Metabolism 、 Cholera toxin 、 Biology 、 Golgi apparatus 、 Ganglioside 、 Cell culture 、 Glycolipid 、 Biophysics 、 Endocrinology
摘要: Abstract Tritium-labeled neoglycolipids consisting of the oligosaccharide ganglioside GM1 attached to cholesterol (GM1OSNH-X-CHOL), phosphatidylethanolamine (GM1OS-PE) and stearylamine (GM1OSNHC18) were synthesized their uptake metabolism by GM1-deficient rat glioma C6 cells determined. When added serum-free culture medium, all three rapidly taken up initially inserted into plasma membrane based on resistance trypsia ability bind cholera toxin. With time, underwent internalization as ratio cell-associated radioactivity cell surface toxin binding increased; this process was slow for GM1OSNH-X-CHOL GM1OS-PE rapid GM1OSNHC18. Analysis lipids extracted from indicated that also GDtaOS-based analogues. In addition, GM1OSNHC18 degraded GM2OS-based analogues, whereas GM2OS-PE not detected. contrast, large amounts 3H recovered in medium treated with label associated material behaved neither an or a neoglycolipid. presence monensin chloroquine, inhibited. Thus, GM1OS-based cells, internalized sorted both Golgi apparatus (sialylated GD1aOS-based analogues) lysosomes (hydrolyzed analogues). The rate extent these processes, however, strongly influenced nature lipid moiety.