Recombinant cellulose crosslinking protein: a novel paper-modification biomaterial

摘要: Cellulose-binding domains have been isolated from various cellulases, and proteins, which lack hydrolytic activity. The hypothesis that a cellulose-binding domain can be used to alter surface mechanical properties of paper was tested. Two Clostriium cellulovorans were fused form cellulose crosslinking protein (CCP). recombinant bifunctional expressed in E. coli, appliedby immersion onto Whatman filter paper, its purified improved the treated paper's (tensile strength, brittleness, Young's modulus energy break). In addition, treatment shown transform into more water-repellent paper. binding under wide range envi-ronmentalconditions, without need for chemical reactions, biodegradability make them attractive moieties design new class paper-modification materials.p>