A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk.
作者： L M Herman , J H De Block , G M Waes
DOI: 10.1128/AEM.61.12.4141-4146.1995
关键词: Spore 、 Lysis 、 Clostridium tyrobutyricum 、 Microbiology 、 Spacer DNA 、 Detection limit 、 Centrifugation 、 Chromatography 、 Raw milk 、 Biology 、 Endospore
摘要: A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial are concentrated by centrifugation after chemical extraction the components. vegetative cells selectively lysed, and their DNA digested washed away. Afterwards, liberated from microwave treatment. For identification C. DNA, a two-step PCR with two nested pairs primers used. were derived 16S-23S rRNA spacer region tyrobutyricum, specificity each them demonstrated. limit can be estimated between 3 30 milk.
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