Characterization of a commercialized SERS-active substrate and its application to the identification of intact Bacillus endospores.

摘要: Surface-enhanced-Raman-spectroscopy (SERS) can be made an attractive approach for the identification of Raman-active compounds and biological materials (i.e., toxins, viruses, or intact bacterial cells spores) through development reproducible, spatially uniform SERS-active substrates. Recently, reproducible (from substrate to substrate), homogeneous (over large areas) SERS-active substrates have been commercialized are now available in the marketplace. Scanning electron microscopy high-resolution, tapping-mode atomic force microscopy have used analyze these novel plasmonic surfaces topographical consistency. Additionally, we assessed, by wavelength-tunable microreflectance spectrometry, spatial distribution localized surface plasmon resonance (LSPR) across a single as well as LSPR λMAX variance from substrate. These analyses reveal that these surfaces topologically with small LSPR Further, we utilized patterned acquire SERS spectral signatures four intact, genetically distinct Bacillus spore species cultivated under identical growth conditions. Salient spectral signature features make it possible discriminate among genetically spores. Additionally, partial least squares, multivariate calibration method, has develop personal-computer-borne algorithms useful classification unknown samples based solely on signatures. To our knowledge, this is first report detailing application these commercially identification intact spores.