Biological Bottom-Up Assembly of Antibody Nanotubes on Patterned Antigen Arrays

摘要: Application of biotechnology in nanofabrication has an advantage to produce functional building-block materials that may not have synthetic counterparts. Here we introduced a new type building block, antibody nanotubes, and demonstrated anchoring them on complementary antigen arrays via antibody-antigen recognition. Biological recognition between the nanotubes permitted recognition-driven assembly ordered nanotube arrays. The array antigens was written by using tip atomic force microscope (AFM) alkylthiol self-assembled monolayer (SAM)-coated Au substrates nanografting. After were immobilized onto shaved regions SAMs with AFM tip, produced incubating antibodies template solutions, selectively attached regions. This technique is very useful when multiple blocks are necessary address specific locations because simultaneous immobilization at binding positions can be achieved single process.