Variant lipolytic enzymes and methods for expression thereof

摘要: The present invention relates to a method for preparing variant lipolytic enzyme comprising expressing in host organism nucleotide sequence which has at least 90% identity with encoding fungal and comprises one modification position corresponds the encoded amino acid a) introduction of glycosylation site compared original enzyme; b) surface location an external loop distal active is more hydrophilic; or c) substitution insertion positions disclosed herein deletion herein. also polypeptide produced by novel nucleic acids.