Proteins, transcripts, and genetic architecture of seminal fluid and sperm in the mosquito aedes aegypti

摘要: The yellow fever mosquito, Aedes aegypti,, transmits several viruses causative of serious diseases, including dengue, Zika, and chikungunya. Some proposed efforts to control this vector involve manipulating reproduction suppress wild populations or replace them with disease-resistant mosquitoes. design such strategies requires an intimate knowledge reproductive processes, yet our basic understanding genetics in remains largely incomplete. To accelerate future investigations, we have comprehensively catalogued sperm seminal fluid proteins (SFPs) transferred females the ejaculate using tandem mass spectrometry. By excluding female-derived isotopic labeling approach, identified 870 280 SFPs. Functional composition analysis revealed parallels known aspects biology SFP function other insects. corroborate proteome characterization, also generated transcriptomes for testes male accessory glands-the primary contributors Ae. aegypti, fluid, respectively. Differential gene expression glands from virgin mated males suggests that transcripts encoding involved protein translation are upregulated post-mating. Several were modulated after mating, but >90% remained unchanged. Finally, a significant enrichment SFPs was observed on chromosome 1, which harbors sex determining locus species. Our study provides comprehensive proteomic transcriptomic characterization production thus foundation investigations biology, functional individual broader examination processes.