Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study.

摘要: A study was conducted to determine the prevalence of Shiga toxin-producing Escherichia coli (STEC) in swine feces United States as part National Animal Health Monitoring System's Swine 2000 study. Fecal samples collected from operations 13 top 17 swine-producing states were tested for presence STEC. After enrichment fecal tryptic soy broth, stx1 and stx2 by use TaqMan E. STX1 STX2 PCR assays. Enrichments positive and/or plated, colony hybridization performed using digoxigenin-labeled probes complementary genes. Positive colonies picked confirmed stx1, stx2, or stx2e genes, isolates serotyped. Out 687 assays, 70% (484 687) toxin 54% (370 687), 64% (436 38% (261 both respectively. 219 that characterized, 29 (13%) produced 14 (6%) 176 (80%) stx2e. Twenty-three contained at least two STEC strains had different serotypes but same genes included a strain possessed addition The belonged various serogroups, including O2, O5, O7, O8, O9, OX10, O11, O15, OX18, O20, O57, O65, O68, O69, O78, O91, O96, O100, O101, O120, O121, O152, O159, O160, O163, O untypeable. It is noteworthy no serogroup O157 recovered. Results this indicate harbor can potentially cause human illness.