Occurrence and ribotypes of Listeria monocytogenes in Gorgonzola cheeses.

作者: Gerardo Manfreda , Alessandra De Cesare , Simone Stella , Massimo Cozzi , Carlo Cantoni

DOI: 10.1016/J.IJFOODMICRO.2004.11.045

关键词: BiologyRibotypingVirulencePopulationFood scienceGenetic diversityFood contaminantSerotypeListeria monocytogenesFood microbiology

摘要: In this study 1656 Gorgonzola cheeses, collected from October 2003 to April 2004 in the same industrial plant located Lombardia (Italy), were analysed order evaluate their level of contamination with Listeria monocytogenes after packaging, as well at end shelf life. A subset isolates was submitted automated EcoRI ribotyping DUP-IDs (DuPont identification library code) and genetic diversity. The isolate profiles included an on-line database named PathogenTracker database. strain similarities between human sporadic strains allowed potential virulence Gorgonzola-associated strains. L. detection rates observed cheese samples monitored packaging life 2.1% 4.8%, respectively. Seventy percent genotyped classified ribotype, labelled 204 S5, indicating that population associated shows a low Ninety belonging II pathogenicity lineage identified for monocytogenes. That includes serotype 1/2a, 1/2c 3c strains, 35% described literature causing listeriosis. Moreover, 16.7% showed similarity >or=99% results incidence cheeses commercialised by tested lower than other Italian blue-veined authors. However, it increased during storage become double life, ranging 30 60 days packaging.

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