Metalloprotein Design and Engineering

摘要: This article covers recent advances in metalloprotein design, focusing on different approaches to their design. Impressive progress has been made designing metal-binding sites peptides, de novo designed proteins, and native protein scaffolds. The design approach can be rational or combinatorial. Under redesigning an existing site a new with dramatically structure function complements well the of by revealing role specific residues responsible for particular structural functional feature interest. To create site, several have used, including based homology, inspection, using automated computer search algorithms, combination above approaches. In addition, modular involving transplanting conserved unit from one into another also shown effective. Design through combinatorial evolutionary methods successful, as it requires little prior knowledge structure. Finally, introducing unnatural amino acids nonnative metal ions/prosthetic groups expand repertoires metalloproteins demonstrated. Successful examples each are given, advantages disadvantages discussed, outlook future research is presented. Keywords: metalloprotein; metallopeptide; protein engineering; protein redesign; de design; modular design; directed evolution; combinatorial design; unnatural acids; phage display