Iron in complex with the alleged phytosiderophore 8-hydroxyquinoline induces functional iron deficiency and non-autolytic programmed cell death in rapeseed plants

摘要: Abstract The alkaloid 8-hydroxyquinoline (HQ) was pointed out as potential phytosiderophore that is taken up unspecifically by iron acquisition strategy I and II plants. HQ in root exudate of the knapweed Centaurea diffusa even considered a factor contributing to invasive success this species. This study compares supply efficiencies Fe–HQ complex Fe–EDTA hydroponic cultures rapeseed (Brassica napus) plant model system explore proposed function. Iron (FeCl3) supplied 2 10 μM concentrations 1:1 ratio with ligand (HQ). After 20 days, Fe content, lipid peroxidation, superoxide anion, hydrogen peroxide, ascorbic acid concentrations, various enzyme activities associated antioxidant defences programmed cell death (PCD), nuclear condensation were determined. form clearly less efficient. These plants developed chlorosis showed symptoms non-autolytic PCD similar those had been subjected deprivation. Reactive oxygen species (ROS) concentration levels (superoxide dismutase, catalase, alkaline protease, caspase-3-like deoxyribonuclease) resembled more observed which suffered from deprivation, than Fe-sufficient results do not support putative function alleged studied range (2–10 μM) but instead corroborate one hypothesis explaining toxicity: released efficiently enough be available for metalloenzymes require it co-factor.