In vitro and in vivo characterization of an ail mutant of Yersinia enterocolitica

摘要: Ail is a 17-kDa protein of Yersinia enterocolitica previously identified on the basis its ability to confer upon Escherichia coli phenotype attachment and invasion cultured epithelial cells. Here we report an examination contribution ail pathogenicity Y. enterocolitica. A low-copy-number plasmid that promoted serum resistance in E. HB101 was constructed. The conferred by affected growth phase culture as well gene copy number. In contrast, number (and relative quantity Ail) found have little effect amount Ail-promoted An mutant constructed characterized vitro. This produced no detectable had reduced invade CHO Serum dependent examined vivo using murine model for infection. identical wild-type strain oral 50% lethal dose studies early colonization Peyer's patches kinetic studies. Western blot (immunoblot) analysis bacteria growing at 48 h postinfection indicated expressed this time point. Thus, our findings confirm contributes phenotypes vitro indicate not required establish infection or cause systemic BALB/c DBA/2 mice.