Taxol Induces the Hematopoietic Growth Factor Granulocyte-Macrophage Colony-stimulating Factor in Murine B-Cells by Stabilization of Granulocyte-Macrophage Colony-stimulating Factor Nuclear RNA

摘要: Taxol, a microtubule-stabilizing agent, has been shown to have anti-neoplastic activity against various tumors. In addition, it that taxol resembles bacterial lipopolysaccharide in its ability activate macrophages. Recently we induces the expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) murine B-cell lines. light similarity and their effects on macrophages, tested whether could also induce GM-CSF present study used B-lymphoma cell line M12.4.1. unstimulated cells, no mRNA was detected, whereas taxol-stimulated cells at concentration 30 µm, induced 4–8 h after stimulation. This induction down-regulated by 10 ng/ml interleukin 4. Actinomycin D chase experiments revealed 4 did not affect half-life taxol-induced cytoplasmic mRNA, nor alter gene transcription. Polymerase chain reaction analysis nuclear RNA, utilizing probes specific for sequences first intron GM-CSF, indicated enhances accumulation precursor RNA decreases this accumulation. The shows novel inducing release hematopoietic growth from B-cells. Since is known recruit macrophages enhance cytotoxicity tumor our observations suggest part antitumor may be due synergistic together with direct cytotoxic actions through microtubule stabilization.