Increased eIF-2 alpha expression in mitogen-activated primary T lymphocytes.
作者： R. B. Cohen , T. R. Boal , B. Safer
DOI: 10.1002/J.1460-2075.1990.TB07601.X
关键词:
摘要: G0 human T cells synthesize protein at low rates and contain very levels of eIF-2 alpha mRNA. plays a pivotal role in the earliest regulated steps translation initiation. We examined gene expression normal stimulated with PHA. Nuclear run-on assays indicate transcription these change 2-fold PHA treatment. Actinomycin D chase experiments show that t1/2 mRNA is similar PHA-treated cells. Analysis nuclear RNA probes specific for intron sequences shows increased after treatment largely due to intranuclear stabilization primary transcript. The increase does not require new synthesis. Hence, this appears be part response program when they are exposed mitogen.
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