Posttranscriptional Lipopolysaccharide Regulation of the Lysozyme Gene at Processing of the Primary Transcript in Myelomonocytic HD11 Cells

摘要: Lysozyme is increasingly expressed in macrophages inflammatory response to bacterial LPS. In this study, we investigated the mechanisms that control expression of lysozyme gene myelomonocytic HD11 cells activated by Nuclear run-on transcription assays showed LPS caused a 15-fold increase rate gene. However, Northern analyses with cDNA and intron sequences revealed LPS-induced nuclear transcripts greatly exceeded rate. Furthermore, untreated t(1/2) <10 min were more unstable than those accumulated LPS-activated cells. We suggested, therefore, increased following treatment was largely due stabilization primary transcript. Interestingly, stability transcript accompanied changes processing including an poly(A) tail length, which gradually shortened after entering cytoplasm. The long tail, however, did not result any polysomal recruitment for translation or cytoplasmic mRNA.