Engineered DNA Polymerases

摘要: This chapter reviews the methods used to generate variants of DNA polymerases that have improved ability, in particular accept unnatural nucleotides, focusing especially on vitro and directed evolution methods. Several natural families independently evolved for millions years their nucleotide substrates with high fidelity ability exclude closely related structures, such as ribonucleoside derivatives. However, can would many applications biotechnology. Directed may be an efficient method produce new capable do so. relies create a library sequence diverse starting gene parent polymerase. These are reviewed here, well examples application variant polymerases. An evolutionary rationalization is offered explain some mutations produced by experiments.